CROI 2015 Program and Abstracts

Oral Sessions

Session TD-B Themed Discussion

Room 615

48 HIV Burden and Biomarker Associations With Colonic HIV RNA During Acute HIV Infection James L. Fletcher 2 ; Trevor A. Crowell 1 ; Robin Dewar 3 ; Irini Sereti 4 ; Bonnie Slike 1 ; Nitiya Chomchey 2 ; Rungsun Rerknimitr 5 ; Nelson L. Michael 1 ; Nicolas Chomont 6 ; Jintanat Ananworanich 1 On behalf of the RV254/SEARCH010 Study Group 1 US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, US; 2 SEARCH, Thai Red Cross AIDS Research Centre, Bangkok, Thailand; 3 Virus Isolation and Serological Lab, National Cancer Institute at Frederick, Frederick, MD, US; 4 National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, US; 5 Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; 6 Vaccine and Gene Therapy Institute Florida, Port St. Lucie, FL, US 49 Identification and Characterization of Individual HIV-Infected CD4 T Cells Ex Vivo Joseph Casazza 1 ; Irene Primmer 1 ; David Ambrozak 1 ; Constantinos Petrovas 1 ; Sara Ferrando- Martinez 1 ; Perla Del Río-Estrada 2 ; Gustavo Reyes-Terán 2 ; Ezequiel Ruiz-Mateos 3 ; John Mascola 1 ; Richard A. Koup 1 1 Vaccine Research Center, NIAID, NIH, Bethesda, MD, US; 2 Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico; 3 HU Virgen del Rocio/IBIS, Sevilla, Spain 50 Efficacy of HIV-1 Monoclonal Antibody Immunotherapy in Acute SHIV- Infected Macaques Diane L. Bolton 1 ; Amarendra Pegu 2 ; KeyunWang 2 ; Kathleen McGinnis 2 ; Kathryn Foulds 2 ; Srinivas Rao 2 ; Merlin L. Robb 1 ; Nelson L. Michael 1 ; John Mascola 2 ; Richard A. Koup 2 1 US Military HIV Research Program, Silver Spring, MD, US; 2 National Institute of Allergy and Infectious Diseases (NIAID), Bethesda, MD, US 51 HIV-1 Infections With Multiple Founders Are AssociatedWith Higher Viral Loads Holly Janes 1 ; SodsaiTovanabutra 2 ; Joshua Herbeck 3 ; Supachai Rerks-Ngarm 4 ; Merlin L. Robb 2 ; Nelson L. Michael 2 ; Peter Gilbert 1 ; Jerome H. Kim 2 ; Morgane Rolland 2 On behalf of the Step/HVTN502 and RV144 study teams 1 Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, US; 2 US Military HIV Research Program, Silver Spring, MD, US; 3 University of Washington, Seattle, WA, US; 4 Thai Ministry of Public Health, Bangkok, Thailand 52 Post-Treatment Controllers Have Particular NK Cells With High Anti-HIV Capacity: VISCONTI Study Daniel Scott-Algara 1 ; Céline Didier 1 ;Vincent Arnold 1 ; Jean-Saville Cummings 1 ; Faroudy Boufassa 2 ; Olivier Lambotte 5 ; Laurent Hocqueloux 4 ; Asier Sáez-Cirión 1 ; Christine Rouzioux 3 1 Institut Pasteur, Paris, France; 2 CESP U1018 Inserm, Le Kremlin-Bicêtre, France; 3 Laboratoire de Virologie, EA 3620–Université Paris Descartes Hôpital Necker, Paris, France; 4 Service des Maladies Infectieuses et Tropicales CHR d’Orléans–La Source, Orleans, France; 5 Hôpital Bicêtre, Le Kremilin-Bicêtre, France 53 Antiretroviral Therapy Preserves Polyfunctional HIV-1 – Specific CD8 T Cells With Stem-Cell –L ike Properties Selena Vigano 1 ; Jordi J. Negron 1 ; Eric S. Rosenberg 2 ; Bruce D.Walker 1 ; Mathias Lichterfeld 2 ; Xu G.Yu 1 1 The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, US; 2 Massachusetts General Hospital, Harvard Medical School, Boston, MA, US 54LB In Vitro Replication and Interferon-Alpha Resistance of Transmitted HIV-1 Variants Zachary Ende 1 ; Martin Deymier 1 ; Angharad Fenton-May 2 ; DanielT. Claiborne 1 ;William Kilembe 3 ; Susan Allen 1 ; Persephone Borrow 2 ; Eric Hunter 1 1 Emory University, Atlanta, GA, US; 2 Oxford University, Oxfordshire, United Kingdom; 3 Zambia Emory HIV Research Project, Lusaka, Zambia 55LB HVTN505 Breakthrough Sequences Show HIV Vaccine-Associated Differences in Env-gp120 Morgane Rolland 1 ; Allan deCamp 2 ; Breana M. Hall 3 ; SodsaiTovanabutra 1 ; Mario Roederer 4 ; Scott M. Hammer 5 ; Magdalena E. Sobieszczyk 5 ; Peter B. Gilbert 2 ; Jerome H. Kim 6 ; James Mullins 3 On behalf of the HVTN505 Sieve Analysis Group 1 US Military HIV Research Program; Henry M. Jackson Foundation for the Advancement of Military Medicine Inc, Silver Spring, MD, US; 2 Fred Hutchinson Cancer Research Center, Seattle, WA, US; 3 University of Washington, Seattle, WA, US; 4 Vaccine Research Center, Bethesda, MD, US; 5 Columbia University, New York, NY, US; 6 US Military HIV Research Program, Silver Spring, MD, US

1:30 pm– 2:30 pm Next Generation of Next-Generation Sequencing Themed Discussion Leader Davey M. Smith , University of California San Diego, San Diego, CA, US 258 PCR-Free Full Genome Characterization of Diverse HIV-1 Strains by Nextgen Sequencing Viswanath Ragupathy 1 ; Feng Gao 2 ; Ana Sanchez 2 ; Marco Schito 3 ;Thomas Denny 2 ; Michael Busch 4 ; Jiangqin Zhao 1 ; Christelle Mbondji 1 ; SaiVikramVemula 1 ; Indira Hewlett 1 1 US Food and Drug Administration, Silver Spring, MD, US; 2 Duke Human Vaccine Institute and Departments of Medicine, Duke University Medical Center, Durham, NC, US; 3 Henry Jackson Foundation, DAIDS, NIAID, Bethesda, MD, US; 4 Blood Systems Research Institute/University of California San Francisco, San Francisco, CA, US Michael G. Berg 1 ; JulieYamaguchi 1 ; Elodie Alessandri-Gradt 2 ; Jean-Christophe Plantier 2 ; Catherine Brennan 1 1 Abbott Laboratories, Abbott Park, IL, US; 2 Virology Unit, National Reference for HIV, Rouen, France 256 Near Full Length HIV-1 Sequencing to Understand HIV Phylodynamics in Africa in Real Time Siva Danaviah; Justen Manasa; EduanWilkinson; Sureshnee Pillay; Zandile Sibisi; Sthembiso Msweli; Deenan Pillay; Tulio de Oliveira University of KwaZulu-Natal, Durban, South Africa 254 Present Applications of a High-Throughput, Single Measure HIV Genomic Incidence Assay SungYong Park 1 ;Tanzy Love 2 ; Nolan Goeken 1 ; Robert Bolan 3 ; Alan S Perelson 4 ; Michael Dube 1 ; Ha Youn Lee 1 1 Keck School of Medicine at University of Southern California, Los Angeles, CA, US; 2 University of Rochester School of Medicine and Dentistry, Rochester, CA, US; 3 Los Angeles Gay and Lesbian Center, Los Angeles, CA, US; 4 Los Alamos National Laboratory, Los Alamos, CA, US 255 A Comprehensive Analysis of Primer IDs to Study Heterogenous HIV-1 Populations David Seifert 1 ; ArminTöpfer 1 ; Francesca Di Giallonardo 2 ; Stefan Schmutz 2 ; Huldrych F. Günthard 2 ;Volker Roth 3 ; Niko Beerenwinkel 1 ; Karin J. Metzner 2 1 ETH Zurich, Basel, Switzerland; 2 University Hospital Zurich, Zurich, Switzerland; 3 University of Basel, Basel, Switzerland 593 Analysis of Resistance Haplotypes Using Primer IDs and Next Gen Sequencing of HIV RNA Valerie F. Boltz 1 ; Jason Rausch 1 ;Wei Shao 2 ; Charles Coomer 1 ; JohnW. Mellors 3 ; Mary Kearney 1 ; John M. Coffin 4 1 National Institutes of Health (NIH), Frederick, MD, US; 2 Leidos, Frederick, MD, US; 3 University of Pittsburgh, Pittsburgh, PA, US; 4 Tufts University, Boston, MA, US Session TD-C Themed Discussion Room 613 1:30 pm– 2:30 pm HIV/CMV Interactions in Transmission and Pathogenesis Themed Discussion Leader Victor Appay , Institut National de la Santé et de la Recherche Médicale, Paris, France 300 Effect of CMV and HIV Replication on T-Cell Exhaustion and Senescence During ART Jennifer M. Dan 1 ; Marta Massanella 1 ; David M. Smith 1 ; Eric S. Daar 2 ; Michael P. Dube 3 ; Richard Haubrich 1 ; Sheldon Morris 1 ; Sara GianellaWeibel 1 1 University of California San Diego, La Jolla, CA, US; 2 Harbor–University of California Los Angeles Medical Center, Torrance, CA, US; 3 University of Southern California Keck School of Medicine, Los Angeles, CA, US 301 HIV Myeloid Derived Suppressor Cells Control Cytomegalovirus 257 Pan-HIV Next-Gen Sequencing Strategy for Viral Surveillance

Tuesday, February 24, 2015 • Oral Sessions

Inflammation by IL-27 Ankita Garg ; Stephen Spector University of California San Diego, La Jolla, CA, US

5

CROI 2015