2019 Ryan White HIV/AIDS Program CLINICAL CONFERENCE

Model of Inflammation

Deeks, Lancet2013

Slide28of43FromSGianellaWeibel,MD atNewOrleans,LA,December4-7,2019,RyanWhiteHIV/AIDSProgramCLINICAL CONFERENCE, IAS  USA.

Low-level Viremia <20 cp/ml is Common During ART

80% Patients had detectable viremia

Median 3.1 copies/ml

Maldarelli F.et al.,PLOSPath, 2007; Palmer S.et al,PNAS, 2008, Lorenzo-Redondo Nature, 2016.

Slide29of43FromSGianellaWeibel,MD atNewOrleans,LA,December4-7,2019,RyanWhiteHIV/AIDSProgramCLINICAL CONFERENCE, IAS  USA.

Yukl et al.

Page12

NIH-PA Author Manuscript NIH-PA Author Manuscript

HIV RNA Is Detectable in GUT During ART

Yukl et al. JID 2010

NIH-PA Author Manuscript

Slide30of43FromSGianellaWeibel,MD atNewOrleans,LA,December4-7,2019,RyanWhiteHIV/AIDSProgramCLINICAL CONFERENCE, IAS  USA.

Figure 2. HIV DNA copy numbers (2A) were measured in peripheral blood mononuclear cells (PBMC) and total gut cells using real time PCR, normalized to total cell numbers by DNA mass (NanoDrop), and normalized to CD4 cells by flow cytometry. Total unspliced HIV RNA levels (2B) were measured using qRT-PCR, normalized to cell numbers by GAPDH, and normalized to CD4 cells by flow cytometry. Whiskers represent the maximum and minimum; upper and lower box borders represent the 75 th and 25 th percentile; the horizontal line indicates the median.

J InfectDis .Authormanuscript; available inPMC2011November15.