CROI 2017 Abstract e-Book

Abstract eBook

Poster and Themed Discussion Abstracts

testing alone (AUC=0.8 vs. 0.75, p=0.007). Adding Dacron-E6/E7 to Dacron-cytology testing improved accuracy 11% for predicting bHSIL over Dacron-cytology alone (AUC=0.83 vs. 0.72, p<0.001), but was no more accurate than Dacron-E6/E7 alone (p=0.78). Paired Dacron-DNA & Dacron-E6/E7 testing improved accuracy 5% over Dacron-DNA alone (AUC=0.81 vs. AUC=0.76, p=0.02); however, Dacron-E6/E7 testing alone was as accurate as paired testing (AUC=0.80 vs. 0.81, p=0.65). Paired Dacron- cytology & Dacron-DNA testing did not improve accuracy over Dacron-cytology (p=0.06) or Dacron-DNA (p=0.97) testing alone. HIV-infected & –uninfected men showed small differences in accuracy across all strategies (Table). However, adding E6/E7 to NF- or Dacron cytology improved accuracy for predicting bHSIL 1.16-1.32-fold over cytology alone (Table). Conclusion: For HIV-infected & –uninfected MSM, hrHPV-E6/E7-mRNA & -DNA testing adds value to cytology screening for predicting bHSIL over cytology alone. Larger studies are needed.

Poster and Themed Discussion Abstracts

593 ANAL HIGH-RISK HPV ASSOCIATIONS WITH SERUM CYTOKINES/CHEMOKINES AND FREE TESTOSTERONE

Dorothy J. Wiley 1 , Hilary K. Hsu 2 , Sitram Vangala 3 , David Elashoff 3 , Xiang Lu 3 , Todd Brown 4 , Gysamber D’Souza 2 , Elizabeth Breen 3 , Stephen Young 3 , Nancy Joste 1 1 Univ of California Los Angeles, Los Angeles, CA, USA, 2 The Johns Hopkins Univ, Baltimore, MD, USA, 3 Tricore Reference Labs, Albuquerque, NM, USA, 4 Univ of NewMexico Hlth Scis Cntr, Albuquerque, NM, USA Background: Inflammatory cytokines and chemokines (biomarkers) prevent infection acquisition or promote clearance. Persistent Group/1-hrHPVs increase risk for anogenital invasive squamous cell carcinomas (SCC) and their precursor lesions, including anal cancers. Anal SCCs are emerging as important non-AIDS defining cancer among HIV-infected gay, bisexual and other men who have sex with men (MSM) and women. Associations between Group/1-hrHPV infections and biomarkers is unclear, especially within the context of HIV coinfection. Methods: Anal-swab specimens for 691 men who have sex with men (MSM) fromMulticenter AIDS Cohort Study were evaluated using cross-sectional analysis. Dacron-swab collected anal cytology specimens were tested for 37 HPVs using PCR and a sensitive linear blot-hybridization assay. Twenty-four inflammatory cytokines/chemokines and free testosterone (sFT) were measured in cryopreserved serum that was collected approximately 24 months before HPV testing. Stepwise logistic regression models selected biomarker candidates (p<0.2) for multivariate analyses. Final analyses compared odds of Group/1-hrHPVs (vs. not) associated with MIP-1β, IFN-γ, and sFT, adjusting for the effects of age, race, smoking, sex-partner number; HIV infection and, among the infected, (HIV) virus load and CD4-T-lymphocyte counts. Results: The median age was 52 years, 79%were HIV-infected, and 62% tested positive for >1 Group/1-hrHPVs. Group/1-hrHPV-infection was associated with MIP-1B, CXCL13/ BLC/BCA-1, IFN-γ cytokines/chemokines in bivariate analyses (p-values<0.05), but not associated with HIV-infection. IFN-γ measurements were substantially lower than population estimates reported using the same technology. Multivariable analysis showed the odds of Group/1-hrHPV infections decreased 1.3-fold with every log10 increase in serumMIP-1β (p=0.01) (Table). Similarly, each log10 increase in sFT increased the odds of detecting Group/1-hrHPVs 1.5-fold (p=0.03) (Table). Positive associations between IFN-γ and Group/1-hrHPVs were suggestive but may not be biologically meaningful (OR=1.4, p=0.06). Conclusion: MIP-1β may protect against infection or promote clearance. sFT is positively associated with Group/1-hrHPV infections. More research is needed.

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