CROI 2015 Program and Abstracts

Poster Listings

388 PD1 Identifies Latently HIV-Infected Nonproliferating and Proliferating CD4 + T Cells Renee M. van der Sluis 1 ; Nitasha A. Kumar 1 ;Vanessa A. Evans 1 ; Rafick P. Sekaly 2 ; Remi Fromentin 2 ; Nicolas Chomont 2 ; Paul U. Cameron 1 ; Sharon R. Lewin 1 1 Doherty Institute, Melbourne University, Melbourne, Australia; 2 VGTI-Vaccine and Gene Therapy Institute Florida, Port St. Lucie, FL, US 389LB 2B4+PD1+ Naïve and Memory CD4+ T Cells Are AssociatedWith Residual Viremia on ART Cynitha Klamar; Feiyu Hong; John Bui; Anthony R. Cillo; Arcadio Agudelo-Hernandez; Deborah A. McMahon; Charles R. Rinaldo; JohnW. Mellors; Bernard J. Macatangay University of Pittsburgh, Pittsburgh, PA, US 390 Nascent LTR-Driven Transcription Can Lead to Translation of HIV Proteins in Resting CD4+ T Cells Laura DeMaster 1 ; Alexander Pasternak 2 ; Una O’Doherty 1 1 University of Pennsylvania, Philadelphia, PA, US; 2 Academic Medical Center University of Amsterdam, Amsterdam, Netherlands

398 The Inducible HIV-1 Reservoir Predicted by Combinations of pre- and on-ART Parameters Anthony R. Cillo ; Michele Sobolowski; Elizabeth Fyne; Dianna Koontz; Feiyu Hong; John W. Mellors University of Pittsburgh, Pittsburgh, PA, US 399 Effects of Antineoplastic Chemotherapy on Dynamics of HIV Population Genetics In Vivo Sarah A. Watters 1 ;Wei Shao 1 ; Kieron Dunleavy 3 ; Margaret Shovlin 3 ; Mark N. Polizzotto 3 ; Thomas S. Uldrick 3 ; RobertYarchoan 3 ;WyndhamWilson 3 ; Frank Maldarelli 1 1 National Cancer Institute (NCI), Frederick, MD, US; 2 National Cancer Institute (NCI), Frederick, MD, US; 3 National Cancer Institute (NCI), Bethesda, MD, US; 4 National Cancer Institute (NCI), Bethesda, MD, US; 5 National Cancer Institute (NCI), Bethesda, MD, US; 6 National Cancer Institute (NCI), Bethesda, MD, US 2:30 pm– 4:00 pm Gene Editing 400 Targeted Disruption of Essential HIV-1 Proviral Genes by Rare-Cutting Endonucleases Harshana S. De Silva Feelixge; Nixon Niyonzima; Harlan L. Pietz; Martine Aubert; Dan Stone; Keith R. Jerome Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, US 401 Enhancing Anti-HIV Gene Therapy: Combining MegaTAL Nuclease Gene EditingWith Selection Cassettes Biswajit Paul 2 ; Alexander Astrakhan 3 ; PatrickYounan 2 ; Blythe D. Sather 4 ; Jordan Jarjour 3 ; Guillermo Romano 2 ; John P. Kowalski 2 ; Iram Khan 4 ; David J. Rawlings 4 ; Hans-Peter Kiem 2 1 Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, US; 2 Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, US; 3 BluebirdBio, Cambridge, MA, US; 4 Seattle Children’s Research Institute, Seattle, WA, US 402 A Phase I Clinical Trial of Autologous CD4 + T Cells ModifiedWith a Retroviral Vector Expressing the MazF Endoribonuclease in Patients With HIV-1 Jeffrey M. Jacobson 1 ; Hideto Chono 2 ; Meghan Metz 1 ; Gabriela Plesa 3 ; Julie Jadlowsky 3 ; Simon Lacey 3 ; Bruce Levine 3 ; HirofumiYoshioka 2 ; Junichi Minemo 2 ; Carl H. June 3 1 Drexel University College of Medicine, Philadelphia, PA, US; 2 Takara Bio Inc., Shiga, Japan; 3 University of Pennsylvania, Philadelphia, PA, US 403 CRISPRs Are Able to Efficiently Target Latent HIV and Halt New Infections Robert Jan Lebbink; Dorien de Jong; FemkeWolters; Emmanuel J.Wiertz; Monique Nijhuis University Medical Center Utrecht, Utrecht, Netherlands Session P-F5 Poster Session Poster Hall 2:30 pm– 4:00 pm HDAC Inhibitors 404 Histone Deacetylase (HDAC) and Histone Acetyltransferase (HAT) Inhibitors Have Opposing Effects on Cellular Susceptibility to HIV Infection Mark B. Lucera 2 ; Curtis Dobrowolski 1 ; Jonathan Karn 1 ; John C.Tilton 2 1 Case Western Reserve University, Cleveland Heights, OH, US; 2 Case Western Reserve University, Cleveland, OH, US 405 Panobinostat Dosing Has Broad but Transient Immunomodulatory Effects in HIV Patients Martin Tolstrup 1 ; Christel R. Brinkmann 1 ;Thomas A. Rasmussen 1 ; Rikke Olesen 1 ; Anne Sofie Kjær 1 ; Mathias Lichterfeld 2 ; Charles Dinarello 3 ; Lars Østergaard 1 ; Ole S. Søgaard 1 1 Aarhus University Hospital, Aarhus, Denmark; 2 Ragon Institute of MIT, MGH and Harvard, Boston, MA, US; 3 University of Colorado, Denver, CO, US TUESDAY, FEBRUARY 24, 2015 Session P-F6 Poster Session Poster Hall

WEDNESDAY, FEBRUARY 25, 2015 Session P-F4 Poster Session

Poster Hall

2:30 pm– 4:00 pm Dynamics of Latency and Reactivation 391 Influenza Vaccination Increases HIV-1 Transcription During Antiretroviral Therapy

Christina C. Yek 1 ; Sara Gianella 1 ; Montserrat Plana 2 ; Pedro Castro 2 ; Felipe Garcia 2 ; Marta Massanella 1 ; David M. Smith 1 1 University of California San Diego, San Diego, CA, US; 2 University of Barcelona, Barcelona, Spain 392 Defective HIV-1 Proviruses Can Be Transcribed Upon Activation Ya-Chi Ho ; Ross Pollack; PatrickYong; Robert F. Siliciano Johns Hopkins University School of Medicine, Baltimore, MD, US 393 Kinetics of HIV-1 Gene Expression Following Reactivation in a Primary Cell Model of Latency VictoriaWalker-Sperling ; Joel Blankson Johns Hopkins University School of Medicine, Baltimore, MD, US 394 Variable HIV Replication Competency Following Latency Disruption in CD4+ T Cells Jason M. Hataye ; Joseph Casazza; David Ambrozak; Eli Boritz;TakuyaYamamoto; Daniel Douek; Richard A. Koup National Institute of Allergy and Infectious Diseases, Bethesda, MD, US 395 Latent HIV-1 Reactivation and Lysosomal Destabilization Synergize to Host Cell Death Metodi Stankov 1 ; Christina Suhr 1 ; Hazel Lin 1 ; Diana Panayotova-Dimitrova 2 ; Christine Goffinet 1 ; Georg Behrens 1 1 Hannover Medical School, Hannover, Germany; 2 Mannheim Clinic of University of Heidelberg, Mannheim, Germany 396 Noninduced Proviral Genome Characterization in Perinatal HIV Infection Kaitlin Rainwater-Lovett 1 ; Carrie Ziemniak 1 ; DouglasWatson 2 ; Katherine Luzuriaga 3 ; Priyanka Uprety 4 ;Yahui Chen 1 ;Ya-Chi Ho 1 ; Deborah Persaud 1 1 Johns Hopkins University School of Medicine, Baltimore, MD, US; 2 University of Maryland, Baltimore, MD, US; 3 University of Massachusetts Medical School, Worcester, MA, US; 4 Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, US; 5 Johns Hopkins University School of Medicine, Baltimore, MD, US 397 Multiple Rounds of T-Cell Activation Induce Additional HIV-1 From the Latent Reservoir

Poster Listings

Nina N. Hosmane ; Adam A. Capoferri; Robert F. Siliciano Johns Hopkins University School of Medicine, Baltimore, MD, US

36

CROI 2015